Friday, 26 December 2008

The Fuzziness of Boundaries-Max Delbrück

In the summer of 1941, a young man-only 34 years old-showed up for the first time at the Cold Spring Harbor Laboratory Campus. He would ultimately become the leader of a generation of scientists who would establish the field of molecular biology. His name was Max Delbrück. Max was born in Berlin. His father Hans, was a professor of history at the University of Berlin and his mother was the grand-daughter of Justus von Liebig (of chemistry fame).

His early interests were directed towards astronomy. However, during the latter part of his graduate studies in Göttingen, the breakthroughs in quantum mechanics caused him to shift to theoretical physics. He obtained his Ph.D in 1930 following which he went back to Berlin and worked as an assistant to Lise Meitner, It was during this time that he discovered the theoretical basis of gamma ray scattering by a Coulomb field, a phenomenon that to this date bears his name, Delbrück scattering.

In 1937, he moved to Caltech, in the United States, where he teamed up with Emory Ellis doing phage research. Together, they demonstrated that viruses reproduce in one step, rather than exponentially (as cellular organisms do). Following the start of World War II, Delbrück chose to remain in the United States, teaching physics at Vanderbilt University while conducting genetic research. His claim to biological fame came with the establishment of the famous "Phage Group" with Salvador Luria and Alfred Hershey at the CSHL . In 1943, he and Luria demonstrated that genetic mutations in bacteria arise in the absence of selection and not as a response to selection. This work was significantly backed up by mathematical models that they developed which were consistent with experimental results. He shared the 1969 Nobel Prize in Medicine and Physiology with Luria.

His name may not be as familiar as Albert Einstein or Charles Darwin, but Max Delbrück was a scientific giant who changed the world through his research in modern biology. He saw no barriers between the sciences.  He had with him his tools from physics and mathematics and the problems from biology. In an essay commemorating Delbrück's 60th birthday, Luria wrote "Seldom has a group been so richly rewarded as have we, the molecular biologist, whom the physicist Max Delbrück, more than anyone else, guided to the explorations of the deep mysteries of life".

In the words of the great man himself

A Max Sing-Along 
(lyrics by Max Delbrück, 1968)

I was born about 100 years ago 
And there is nothing in this world that I don't know
I have Mendel in the garden
And I steered the ship for Darwin
I showed Otto how the neutrons had to go!

I had Watson grab the helix by the tail
I told Morgan that the flies would never fail
And the prize that went to Feynman
Well the theory was mine man,
And my song and inspiration never stale!

And, my buddy, Linus owes a debt to me,
For the structure of his modern chemistry,
Of the phage I am the father,
But I didn't want the bother,
Of collecting data so statistic-al-ly!

I'm a single-minded scientific man....

Thursday, 25 December 2008

The Fuzziness of Boundaries-Introduction

How times change!! A few years back, when I announced my plan of pursuing a research career in fundamental biological sciences, I was labeled by many as a mediocre student who did not want to pursue a degree in engineering for want of insight in the fields of physics, mathematics and engineering. Just a week ago, I was with a colleague (he is an electronics engineer studying at the EPFL) in Lausanne and our discussion soon turned to the evergreen topic of biological neural networks. I impressed upon him, the significance of the ongoing Blue Brain project at the Brain Mind Institute in the EPFL. 

The project uses two huge IBM Blue Gene supercomputers to simulate a neocortical column (the basic functional unit of the brain, containing 10,000 neurons). At the push of a button, the model could reconstruct biologically accurate neurons and automatically connect them in a biological manner, a task than involves positioning 30 million synapses (connections that one neuron makes with another, edges between nodes, if you happen to be a graph theorist). All very well except for one tiny detail. The brain happens to consist of 100 billion neurons and 100 trillion synapses!!! Heck, the Blue Brain doesn't come even remotely close to the actual situation. And it requires two huge supercomputers just for a mere 10,000. 

Is the Blue Brain Project a sham then? The answer is no. It is an engineering feat and it is here to provide more clues on one of the most profound questions in biology, the mammalian brain. The point I'm trying to make here is that physical sciences, engineering sciences and the biological sciences were never mutually exclusive. Biology brings the problems. Big problems, trust me. The physical sciences bring with it the theory, principles and the foundation needed to tackle them. Finally, the engineering sciences bring the tools. 

Having said all of this, in the next part of this series, I shall introduce some great men of science and their efforts in breaking the wall between fields, such that today, I have no idea where one ends and the other starts. The boundaries are seemingly fuzzed up and are getting fuzzier by the day.

Sunday, 7 September 2008

The International Genetically Engineered Machines Competition

Last November, I was part of the team from the National Center for Biological Sciences (NCBS), Bangalore, India, at iGEM, which is an annual contest held at the Massachussets Institute of Technology (MIT), USA

(From R to L, Krishna, Mukund and Varun with the trophy)

What is iGEM?

iGEM addresses the question: Can simple biological systems be built from standard interchangeable parts and operated in living cells? Or is biology simply too complicated to be engineered this way? The only way to try and answer this question is to actually engineer biological devices. This forms the basis for the new and emerging field of Synthetic Biology 

NCBS won the "Best Model and Simulation Award". For more details of our project, visit the NCBS iGEM Team-2007 Wiki